Annexin A5 Antibody

Product Specifications

Background

The Annexin family is composed of at least thirteen mammalian genes (Annexin A1-13) . These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. Annexins have roles in membrane fusion, endocytosis, secretion, and repair. Annexin A1 binds to cellular membranes in a calcium-dependent manner, promotes membrane fusion and endocytosis, and has been implicated as an anti-inflammatory mediator. Annexin A2 is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A5 is a PKC inhibitor, directly interacts with VEGFR2 receptor, and binds phosphatidylserine to inhibit blood coagulation. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.

Synonyms

Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, CBP-I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, PP4, Placental anticoagulant protein I, PAP-I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, VAC-alpha, ANXA5, ANX5, ENX2, PP4

Swiss Prot

P08758

Host

Mouse

Cross Reactivity

Human

Target

Annexin A5

Clonality

Monoclonal

Isotype

IgG1

Clone

M017

Conjugation

Unconjugated

Source

Clone M017 was generated from a proprietary antigen related to human Annexin A5 in the MDA-MB-231 breast cancer cell line.

Applications

WB, IP

Purification

Purified by Protein G.

Dilution

WB (1:300-5000), IP (1-2ug)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Clone M017 detects a 35 kDa* protein corresponding to the molecular mass of Annexin A5 on SDS-PAGE immunoblots of human A431, MDA-MB-231, MeWo, and PC3 cells, as well as human skin, breast, lung, and brain. The antibody works for ELISA, western blot, and immunoprecipitation. In addition, mass spectrometry analysis of immunoprecipitates using AM0171 in human MDA-MB-231 cell lysates confirms this antibody only detects Annexin A5 protein.