Syntaxin Antibody

Product Specifications

Background

Syntaxins are a family of proteins involved in the docking of synaptic vesicles with the plasma membrane. Syntaxin, along with synaptobrevin and SNAP-25, forms the SNARE complex which mediates synaptic vesicle fusion and exocytosis. Binding of Munc18-1 to the N-terminus of syntaxin 1 has been demonstrated to be essential for exocytic membrane fusion (Khvotchev et al., 2007) . Decreased levels of phosphorylated syntaxin 1 in the prefrontal cortex have recently been correlated with schizophrenia (Castillo et al., 2010) .

Synonyms

HPC 1 antibody, Neuron specific antigen HPC1 antibody, Neuron-specific antigen HPC-1 antibody, OTTHUMP00000174615 antibody, OTTHUMP00000174616 antibody, OTTHUMP00000174617 antibody, OTTHUMP00000174618 antibody, P35-1 antibody, STX1 antibody, STX1A antibody, STX1A_HUMAN antibody, SYN1A antibody, Syntaxin 1A (brain) antibody, Syntaxin 1A brain antibody, Syntaxin-1A antibody

Gene ID

6804

Swiss Prot

Q16623

Host

Mouse

Cross Reactivity

Human, Rat, Pig, Hamster

Target

Syntaxin

Clonality

Monoclonal

Isotype

IgG1

Clone

SP8

Conjugation

Unconjugated

Source

Synaptic immunoprecipitate (crude) from human brain.

Applications

WB, IHC

Purification

Purified by Protein G.

Concentration

Lot Dependent

Dilution

WB (1:300-5000), IHC ()

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~36 kDa syntaxin protein. The antibody has also been used for immunohistochemistry on formalin fixed, vibratome sections. *Does not work on paraffin sections.