SHARPIN Polyclonal Antibody
Product Specifications
Background
Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.
Synonyms
SIPL1; Sharpin; Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1; hSIPL1; PSEC0216
Gene ID
81858
Swiss Prot
Q9H0F6
Cellular Locus
Cytoplasm
Host
Rabbit
Cross Reactivity
Mouse
Immunogen
271-387/387
Target
SHARPIN
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human SHARPIN
Applications
WB, IHC-P, IF (IHC-P)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), IHC-P (1:200-400), IF (IHC-P) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
81858
Predicted Cross Reactivity
Human, Rat, Dog
Available Sizes
Curated Selection
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