Phosphothreonine Antibody

Product Specifications

Background

Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity

Synonyms

Phosphoser/thr mAb

Modification Site

PThr

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Anti-Phosphothreonine

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Phosphothreonine synthetic peptides coupled to carrier protein.

Applications

WB, IP

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000), IP (1-2ug)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

This antibody was cross-adsorbed to unphosphorylated peptide then Affinity purification using phospho-threonine peptide (without carrier) . The antibody detects many serine or threonine phosphorylated proteins by western blot and ELISA.