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Phospho-14-3-3 protein zeta (Ser58) Polyclonal Antibody, RBITC Conjugated

Product Specifications

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions.

Synonyms

YWHAEphospho S58; YWHAZphospho S58; 14 3 3; 14 3 3 protein beta; 14 3 3 protein beta/alpha; 14 3 3 protein zeta; 14-3-3 protein beta/alpha; KCIP 1; Protein 1054; Protein kinase C inhibitor protein 1; YWHAB; YWHAZ; GW128; HS1; KCIP-1; YWHAE; YWHAA; 1433Z_HUMAN; Full=14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1.

Gene ID

7534, 7529, 10971

Modification Site

Ser58

Cellular Locus

Cytoplasm

Host

Rabbit

Cross Reactivity

Human

Target

Phospho-14-3-3 protein zeta (Ser58)

Clonality

Polyclonal

Isotype

IgG

Conjugation

RBITC

Source

KLH conjugated synthetic phosphopeptide derived from human 14-3-3 protein zeta/delta around the phosphorylation site of Ser58 [RS (p-S) WR]

Applications

WB, FCM

Purification

Purified by Protein A.

Excitation Emission

570nm/595nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.biossantibodies.com/datasheets/bs-3000R-RBITC

Specificity

This antibody will react with all 14-3-3 proteins containing the conserved motif RS (S-p) WR.

Gene ID URL

7534, 7529, 10971

Predicted Cross Reactivity

Mouse, Rat, Dog, Cow, Horse, Chicken, Rabbit

Curated Selection

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