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Phospho-BAD (Ser128) Polyclonal Antibody, PerCP-Cy5.5 Conjugated

Product Specifications

Background

Promotes cell death. Successfully competes for the binding to Bcl-X (L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X (L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Synonyms

Bbc2; AI325008; Bcl2-associated agonist of cell death; BAD; Bcl-2-binding component 6; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; Bbc6

Gene ID

12015

Swiss Prot

Q61337

Modification Site

Ser128

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-BAD (Ser128)

Clonality

Polyclonal

Isotype

IgG

Conjugation

PerCP-Cy5.5

Source

KLH conjugated synthetic phosphopeptide derived from mouse BAD around the phosphorylation site of Ser128

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

482nm/694nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.biossantibodies.com/datasheets/bs-0893R-PerCP-Cy5.5

Specificity

This phophorylation site is homologous to that of Ser129 in Rat.

Gene ID URL

12015

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