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Phospho-AKT (Ser473) Polyclonal Antibody, AbBy Fluor™ 680 Conjugated

Product Specifications

Background

AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Synonyms

Akt; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha; Akt1

Gene ID

24185

Swiss Prot

P47196

Modification Site

Ser473

Cellular Locus

Cytoplasm, Nucleus, Cell membrane

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-AKT (Ser473)

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 680

Source

KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473

Applications

WB, IF

Purification

Purified by Protein A.

Excitation Emission

679nm/702nm

Concentration

1µg/µl

Dilution

WB (1:500-2000), IF (1:100-500)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.biossantibodies.com/datasheets/bs-0876R-BF680

Gene ID URL

24185

Available Sizes

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