BUB3 Recombinant Antibody
Product Specifications
Background
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Synonyms
Mitotic checkpoint protein BUB3; BUB3
Gene ID
9184
Swiss Prot
O43684
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Bub3
Clonality
Recombinant
Conjugation
Unconjugated
Source
Recombinant protein corresponding to the N-terminus of human Bub3.
Applications
WB, FCM, IHC-P, IF (IHC-P), IF (ICC), IP
Purification
Purified by Protein A.
Concentration
1ug/ul
Dilution
WB (1:300-5000), FCM (1:20-100), IHC-P (1:200-400), IF (IHC-P) (1:50-200), IF (ICC) (1:50-200), IP (1-2ug)
Buffer
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
9184
Isotype
IgG
Curated Selection
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