E2F3 Polyclonal Antibody
Product Specifications
Background
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (By similarity) .
Synonyms
E2F-3; Transcription factor E2F3; E2F3; KIAA0075
Gene ID
1871
Swiss Prot
O00716
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Mouse
Immunogen
381-465/465
Target
E2F3
Clonality
Polyclonal
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human E2F3
Applications
WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1ug/ul
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Prediction Reactivity
Human, Rat, Dog, Cow, Pig, Horse, Rabbit
Gene ID URL
1871
Isotype
IgG
Curated Selection
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