SPARC Polyclonal Antibody

Product Specifications

Background

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca (2+) with a low affinity and an EF-hand loop that binds a Ca (2+) ion with a high affinity.

Synonyms

ON; SPARC; Basement-membrane protein 4; BM-4; Osteonectin; Secreted protein acidic and rich in cysteine

Gene ID

6678

Swiss Prot

P09486

Cellular Locus

Secreted, Cell membrane, Extracellular matrix

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Immunogen

101-200/303

Target

SPARC

Clonality

Polyclonal

Conjugation

Unconjugated

Source

KLH conjugated synthetic peptide derived from human SPARC

Applications

WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Concentration

1ug/ul

Dilution

WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Prediction Reactivity

Dog, Cow, Pig, Horse, Rabbit

Gene ID URL

6678

Isotype

IgG

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