SPATA17 Antibody / Spermatogenesis-associated protein 17
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
Q96L03
Host
Rabbit
Reactivity
Human
Immunogen
E.coli-derived human SPATA17 recombinant protein (Position: M1-Q338) was used as the immunogen for the SPATA17 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This SPATA17 antibody is available for research use only.
Storage Conditions
After reconstitution, the SPATA17 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Product Datasheet
http://www.nsjbio.com/tds-pdf/spata17-antibody-spermatogenesis-associated-protein-17-fy12290-nsj-bioreagents
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the SPATA17 antibody should be determined by the researcher.
Image Legend
Flow Cytometry analysis of PC-3 cells using anti-SPATA17 antibody. Overlay histogram showing PC-3 cells stained with (Blue line) . To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPATA17 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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