MUC-1 Antibody

MAb VU-2G7 reacts with MUC1/Mucin-1/Epithelial Marker Antigen/EMA, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of VU-2G7 has not been established with epitope fingerprinting. The extra cellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.

Product Specifications

Specifications

ELISA: order BSA/sodium azide-free format for coating

UniProt

P15941

Host

Mouse

Reactivity

Human

Immunogen

A synthetic glycosylated MUC1 60mer tandem repeat NH2- (HGVTSAPDT (GalNAc) RPAPGSTAPPAHG) 3- COOH was used as the immunogen for the MUC-1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

VU-2G7

Applications

ELISA

Purity

Protein G affinity chromatography

Format

Purified

Limitations

This MUC-1 antibody is available for research use only.

Storage Conditions

Store the MUC-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the MUC-1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min