IGLV1-51 Antibody / Lambda Light Chain

This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P01701

Host

Mouse

Reactivity

Human

Immunogen

Purified human lambda light chain was used as the immunogen for the was used as the immunogen for the IGLV1-51 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

IGLC1/9475

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Limitations

This IGLV1-51 antibody is available for research use only.

Storage Conditions

Aliquot the IGLV1-51 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the IGLV1-51 antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic and secreted

Image Legend

IHC staining of FFPE human tonsil tissue with IGLV1-51 antibody (clone IGLC1/9475) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.