Recombinant CA9 Antibody / Carbonic anhydrase 9

Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200) .In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

Q16790

Host

Mouse

Reactivity

Human

Immunogen

The extracellular N-terminal proteoglycan-like region of the human protein was used as the immunogen for the recombinant CA9 antibody. The epitope is localized to the amino acids 80-100 region.

Clonality

Recombinant Monoclonal

Isotype

IgG2b κ

Clone

RCA9/9937

Applications

IHC-P, WB

Purity

Protein G affinity

Format

Purified

Limitations

This recombinant CA9 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant CA9 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant CA9 antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic, nuclear

Image Legend

Western blot testing of human stomach tissue lysate with recombinant CA9 antibody (clone rCA9/9937) . Predicted molecular weight: 50-55 kDa but may be observed at higher molecular weights due to glycosylation.