Anti-eEF1A2 (Ser358) Antibody

Our Anti-eEF1A2 (Ser358) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse eEF1A2 (Ser358) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Specifications

Background

Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle (Knudsen et al., 1993) . Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors (Anand et al., 2002) . Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites (Soares et al., 2009) . Ribosome-associated JNK phosphorylates Ser-358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome (Gandin et al., 2013) .

Synonyms

EEF1A-2 antibody, Eef1a2 antibody, EEF1AL antibody, EF 1 alpha 2 antibody, EF-1-alpha-2 antibody, EF1A antibody, EF1A2_HUMAN antibody, Elongation factor 1 A 2 antibody, Elongation factor 1 alpha antibody, Elongation factor 1 alpha 2 antibody, Elongation factor 1-alpha 2 antibody, Eukaryotic elongation factor 1 A-2 antibody, Eukaryotic translation elongation factor 1 alpha 2 antibody, FLJ41696 antibody, HS1 antibody, OTTHUMP00000031776 antibody, Statin like antibody, Statin S1 antibody, Statin-S1 antibody, STN antibody, STNL antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

EEF1A2

NCBI Gene ID

1917

UniProt

Q05639

Modification Site

Ser358

Host

Rabbit

Reactivity

Human and Mouse

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 of human eEF1A2, conjugated to keyhole limpet hemocyanin (KLH) .

Immunogen Type

Human

Target

EEF1A2 (Ser358)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Concentration

Lot Dependent

Format

Antigen Affinity Purified from Pooled Serum

Form

Liquid

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylated

Molecular Weight

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

WB Dilution

1:1000

Specificity

Specific for endogenous levels of the ~50 kDa eEF1A2 phosphorylated at Ser358. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Formulation

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Quality Control

Western blots performed on each lot.

Species

Chicken, Non-Human Primate, Rat, Sheep, Xenopus

Isotype

IgG

Curated Selection

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