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Anti-Actin Antibody

Our Anti-Actin primary antibody from PhosphoSolutions is mouse monoclonal. It detects all known species' Actin and is ascites fluid. It is great for use in WB, IHC, ICC.

Product Specifications

Background

Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.

Synonyms

A actin antibody, ACTA antibody, ACTA1 antibody, Actin alpha skeletal muscle antibody, Actin antibody, actin alpha 1 skeletal muscle 1 antibody, actin alpha 1 skeletal muscle antibody, Actin alpha skeletal muscle antibody, actina antibody, actine antibody, ACTS_HUMAN antibody, aktin antibody, Alpha Actin 1 antibody, Alpha skeletal muscle Actin antibody, alpha skeletal muscle antibody, alpha-actin antibody, Alpha-actin-1 antibody, ASMA antibody, CFTD antibody, CFTD1 antibody, CFTDM antibody, MPFD antibody, NEM1 antibody, NEM2 antibody, NEM3 antibody, nemaline myopathy type 3 antibody

Other Statements

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Gene Name

ACTA1

NCBI Gene ID

421534

UniProt

P68139

Host

Mouse

Reactivity

All Known Species

Immunogen

Preparation of chicken gizzard actin

Immunogen Type

Chicken

Target

Actin

Clonality

Monoclonal

Clone

C4

Conjugation

Unconjugated

Applications

ELISA, WB, IHC, ICC

Concentration

1 mg/ml

Format

Mouse ascites fluid

Form

Liquid

Buffer

Ascites fluid + 0.01% NaN3.

Molecular Weight

42

Shipping Conditions

Blue Ice

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Datasheet

https://cdn.shopify.com/s/files/1/0520/9063/4413/files/125-ACT.pdf

IHC Dilution

1:500

WB Dilution

1:5000

Specificity

Specific for endogenous levels of the ~42 kDa actin protein. Reacts with all actin isoforms.

Formulation

Unpurified ascites fluid.

ICC Dilution

1:200-1:500

Quality Control

Western blots performed on each lot.

Isotype

IgG1

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