Anti-Actin Antibody
Our Anti-Actin primary antibody from PhosphoSolutions is mouse monoclonal. It detects all known species' Actin and is ascites fluid. It is great for use in WB, IHC, ICC.
Product Specifications
Background
Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.
Synonyms
A actin antibody, ACTA antibody, ACTA1 antibody, Actin alpha skeletal muscle antibody, Actin antibody, actin alpha 1 skeletal muscle 1 antibody, actin alpha 1 skeletal muscle antibody, Actin alpha skeletal muscle antibody, actina antibody, actine antibody, ACTS_HUMAN antibody, aktin antibody, Alpha Actin 1 antibody, Alpha skeletal muscle Actin antibody, alpha skeletal muscle antibody, alpha-actin antibody, Alpha-actin-1 antibody, ASMA antibody, CFTD antibody, CFTD1 antibody, CFTDM antibody, MPFD antibody, NEM1 antibody, NEM2 antibody, NEM3 antibody, nemaline myopathy type 3 antibody
Other Statements
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Gene Name
ACTA1
NCBI Gene ID
421534
UniProt
P68139
Host
Mouse
Reactivity
All Known Species
Immunogen
Preparation of chicken gizzard actin
Immunogen Type
Chicken
Target
Actin
Clonality
Monoclonal
Clone
C4
Conjugation
Unconjugated
Applications
ELISA, WB, IHC, ICC
Concentration
1 mg/ml
Format
Mouse ascites fluid
Form
Liquid
Buffer
Ascites fluid + 0.01% NaN3.
Molecular Weight
42
Shipping Conditions
Blue Ice
Storage Conditions
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Product Datasheet
https://cdn.shopify.com/s/files/1/0520/9063/4413/files/125-ACT.pdf
IHC Dilution
1:500
WB Dilution
1:5000
Specificity
Specific for endogenous levels of the ~42 kDa actin protein. Reacts with all actin isoforms.
Formulation
Unpurified ascites fluid.
ICC Dilution
1:200-1:500
Quality Control
Western blots performed on each lot.
Isotype
IgG1
Curated Selection
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