Raf1 Rabbit Polyclonal Antibody
Product Specifications
Background
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.
Product Name Alternative
RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
Gene Name
RAF1
Gene ID
5894
Swiss Prot
P04049
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
WB, IHC, ICC/IF, ELISA
Field of Research
Signal Transduction
Purification
Affinity Purification
Dilution
WB 1:500-1:1000, IHC 1:50-1:100, ICC/IF 1:50-1:200, ELISA 1:5000-1:20000
Form
Liquid
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Modification
Unmodified
Molecular Weight
Calculated MW: 73 kDa; Observed MW: 73 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.
Isotype
IgG
Available Sizes
Curated Selection
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