P16 INK4a polyclonal antibody
Product Specifications
Background
The progression of cells through the cell cycle is regulated by a family of proteins designated cyclin-dependent kinases (Cdks) . Sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. Multiple proteins are encoded by the tumor suppressor gene CDKN2A (MTS1/ p16INK4a) via translation of alternate reading frames, resulting in the production of the p19 ARF protein in mice and the p14 ARF protein in humans. p14 ARF induces an increase in MDM2 and p21 levels and leads to cell cycle arrest in both G1 and G2/M. p14 ARF is negatively regulated by p53 and is known to bind directly to MDM2. CDKN2A also encodes the mitotic protein p16, which binds to and inhibits the Cdk4/cyclin D complex.
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
CDKN2; MTS1; Cyclin-dependent kinase inhibitor 2A isoforms 1/2/3; Cyclin-dependent kinase 4 inhibitor A; CDK4I; Multiple tumor suppressor 1; MTS-1; p16-INK4a; p16-INK4; p16INK4A
Swiss Prot
P42771
Reactivity
Human, Mouse, Rat
Immunogen
KLH-conjugated synthetic peptide of human p16 INK4a.
Conjugation
Unconjugated
Applications
WB, IHC, IF/ICC
Dilution
WB: 1:500~1:2000 IHC: 1:50~1:200 IF/ICC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 17 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P16 INK4a pAb detects endogenous levels of p16 INK4a protein.
Applications Notes
Western blot analysis of p16 INK4a expression in HeLa (A), MCF7 (B) whole cell lysates.
Host or Source
Rabbit
Available Sizes
Curated Selection
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