PMSF

PMSF (Phenylmethylsulfonyl fluoride) is a nonspecific and irreversible serine proteases inhibitor. PMSF inhibits some thiol proteases, non-protease enzymes, and acetylcholinesterase. PMSF can be used to prepare protein extracts from tissue and cell lysates[1][2][3][4].

Product Specifications

CAS Number

[329-98-6]

Product Name Alternative

Phenylmethylsulfonyl fluoride; Benzylsulfonyl fluoride

UNSPSC

12352005

Hazard Statement

H225, H315, H319

Target

Cathepsin; Ser/Thr Protease

Type

Reference compound

Related Pathways

Metabolic Enzyme/Protease

Field of Research

Neurological Disease

Assay Protocol

https://www.medchemexpress.com/pmsf.html

Purity

99.49

Solubility

DMSO : 250 mg/mL (ultrasonic) |H2O : < 0.1 mg/mL|Isopropyl alcohol : 60 mg/mL (ultrasonic)

Smiles

O=S(CC1=CC=CC=C1)(F)=O

Molecular Formula

C7H7FO2S

Molecular Weight

174.19

Precautions

H225, H315, H319

References & Citations

[1]Sekar, M.C. and B.D. Roufogalis, Differential effects of phenylmethanesulfonyl fluoride (PMSF) on carbachol and potassium stimulated phosphoinositide turnover and contraction in longitudinal smooth muscle of guinea pig ileum. Cell Calcium, 1984. 5 (3) : p.|[2]Guther, M.L., W.J. Masterson, and M.A. Ferguson, The effects of phenylmethylsulfonyl fluoride on inositol-acylation and fatty acid remodeling in African trypanosomes. J Biol Chem, 1994. 269 (28) : p. 18694-701.|[3]Compton, D.R. and B.R. Martin, The effect of the enzyme inhibitor phenylmethylsulfonyl fluoride on the pharmacological effect of anandamide in the mouse model of cannabimimetic activity. J Pharmacol Exp Ther, 1997. 283 (3) : p. 1138-43.|[4]Asadi Akbarabadi E, et a. PMSF Attenuates Morphine Antinociceptive Tolerance and Dependence in Mice: Its Association with the Oxidative Stress Suppression. Iran J Pharm Res. 2021 Summer;20 (3) :300-309.