D-Luciferin, Potassium Salt (Proven and Published®)

Tested and Certified forin vivoimaging (See "Luciferin FAQ" in technical documentation) GoldBio’s D-Luciferin Potassium Salt is a highly pure, water-soluble substrate optimized for bioluminescence imaging in luciferase-expressing systems. As the essential reagent for firefly luciferase-basedin vivoimaging, this compound enables researchers to non-invasively monitor gene expression, tumor progression, stem cell fate, and infectious disease spread with exceptional sensitivity. With over two decades of proven application in transgenic animal models and high-throughput assays, D-Luciferin Potassium Salt provides unmatched brightness, low background, and consistent performance. Ideal for longitudinal imaging, drug screening, and functional genomics, this bioluminescent substrate is a critical tool in modern molecular biology and biomedical research. Backed by peer-reviewed citations and validated in published protocols, GoldBio’s D-Luciferin is the substrate of choice for researchers seeking high-quality reagents for reproducible and publication-ready imaging."LUCK" and "Proven and Published" are Registered trademarks in the U.S. Patent and Trademark Office.Request a Bulk QuoteNeed larger quantities for your research or production runs? Usethis form to request a custom bulk quoteand secure the best value for your lab. Your account manager will follow up with you promptly to provide tailored pricing and support for your order.Research Applications for D-Luciferin Potassium Salt (Click each for more information) In Vivo Bioluminescence Imaging (BLI) Purpose: D-Luciferin is the essential substrate for non-invasive imaging of luciferase-expressing cells, tissues, or organisms in live animal models.How It Works: Upon intraperitoneal injection, D-Luciferin is oxidized by firefly luciferase in the presence of ATP, Mg²⁺, and oxygen, producing bioluminescence (~560 nm) detectable by CCD cameras.Applications: Real-time monitoring of tumor growth, gene expression, disease progression, and therapeutic efficacy.Inoue, Y., Izawa, K., Kiryu, S., Tojo, A., & Ohtomo, K. (2009) . Comparison of subcutaneous and intraperitoneal injection of D-luciferin for bioluminescence optical imaging. European Journal of Nuclear Medicine and Molecular Imaging, 36 (5), 821–829.Pathogen Infection Models in AnimalsPurpose: Enables real-time imaging and quantification of luciferase-expressing pathogens in living hosts.How It Works: Pathogens genetically modified to express firefly luciferase are tracked via bioluminescence using D-Luciferin administration, allowing dynamic visualization of infection burden and treatment response.Applications: Monitoring pathogen dissemination, antibiotic efficacy, and host-pathogen interactions.Andreu, N., Zelmer, A., Fletcher, T., Elkington, P. T., Ward, T. H., Ripoll, J., Parish, T., Bancroft, G. J., Schaible, U., Wiles, S., & Williams, A. (2010) . Optimization of bioluminescent reporters for use with mycobacteria. PLoS ONE, 5 (5), e10777.Stem Cell Tracking and Regenerative MedicinePurpose: Allows long-term monitoring of viability and localization of transplanted stem or progenitor cells in vivo.How It Works: Luciferase-labeled stem cells emit light upon D-Luciferin administration, enabling repeated non-invasive imaging to assess survival, engraftment, and migration.Applications: Tissue regeneration, spinal cord repair, cardiac therapy, and neural regeneration research.Conway, M., Xu, T., Kirkpatrick, A., Ripp, S., Sayler, G., & Close, D. (2020) . Real-time tracking of stem cell viability, proliferation, and differentiation with autonomous bioluminescence imaging. BMC Biology, 18, 79.Transgenic Reporter Gene AssaysPurpose: Measures promoter activity and gene expression dynamics using luciferase as a reporter.How It Works: Luciferase placed under regulatory control of a promoter of interest generates luminescence upon D-Luciferin administration, quantifying gene activation in real time.Applications: Circadian rhythm studies, gene regulation, toxicology, and signal transduction research.Zhang, Y., Bressler, J. P., Neal, J., Keck, J. G., & Malarkey, D. E. (2007) . ABCG2/BCRP expression modulates D-luciferin-based bioluminescence imaging. Cancer Research, 67 (19), 9389–9397.High-Throughput Screening (HTS) Using Luciferase Reporter AssaysPurpose: Facilitates scalable screening of compounds affecting cellular pathways via luminescent readouts.How It Works: D-Luciferin functions as the substrate for luciferase-based assays in multiwell formats; luminescence output reflects compound effects on gene or protein activity.Applications: Drug discovery, pathway activation/inhibition assays, functional genomics.Inglese, J., Auld, D. S., Jadhav, A., Johnson, R. L., Simeonov, A., Yasgar, A., Zheng, W., & Austin, C. P. (2006) . Quantitative high-throughput screening: A titration-based approach that efficiently identifies biological activities in large chemical libraries. Proceedings of the National Academy of Sciences, 103 (31), 11473–11478.BenefitsNon-invasive quantification: Enables longitudinal, real-time tracking of biological events in living systems without tissue harvesting.High signal-to-noise ratio: Produces low background with strong luminescence, ideal for sensitive detection.Proven versatility: Used across oncology, infectious disease, stem cell, and neuroscience models.Compatible with transgenic luciferase lines and cell lines: Broad experimental flexibility.Stable and highly soluble: Reliable substrate preparation for in vivo and in vitro imaging applications.