EIF4E (Phospho-Ser209) Antibody
This is a eIF4E (Phospho-Ser209) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.
Product Specifications
Background
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Synonyms
MRNA cap-binding protein; eIF-4F 25 kDa subunit
Gene ID
1977
Swiss Prot
P06730
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 209 (S-G-S (p) -T-T) derived from Human eIF4E.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Phospho-Specific Antibody
Applications
WB, IHC, IF
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Assay Type
Antibody
Concentration
1.0 mg/mL
Dilution
WB 1:500-1:1000, IHC 1:50-1:100, IF 1:100-1:200
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles.
Host or Source
Rabbit
Isotype
IgG
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
