Recombinant PHF8 Monoclonal Antibody
Product Specifications
Background
PHF8 is a histone lysine demethylase that functions as a transcriptional activator by specifically demethylating a number of repressive histone methylation marks: mono- and Dimethyl-histone H3 Lys9 (H3K9me1 and H3K9me2), Dimethyl-histone H3 Lys27 (H3K27me2) and Monomethyl-histone H4 Lys20 (H4K20me1) . PHF8 contains an N-terminal zinc finger-like PHD domain that binds Trimethylated histone H3 Lys4 (H3K4Me3) and a C-terminal jumonji domain that is responsible for the demethylase activity. PHF8 is highly expressed in prostate cancer, laryngeal squamous cell carcinoma, and human non-small-cell lung cancer (NSCLC) . Its expression is predictive of poor survival. Overexpression of PHF8 increases cell proliferation and cell motility, while silencing of PHF8 reduces cell proliferation, migration, and invasion.
Abbreviation
PHF8
UniProt
Q9UPP1
Host
Rabbit
Reactivity
Human; Rat; Mouse
Immunogen
Recombinant human PHF8 fragment
Target
ZNF; Phf; KIAA; KIAA1111; ZNF422; PHF8
Clonality
Monoclonal,Recombinant
Clone
A514
Conjugation
Unconjugated
Applications
WB
Field of Research
Epigenetics and Nuclear Signaling
Purification
Protein A purified
Concentration
1 mg/mL
Dilution
WB 1:500-1:1000
Buffer
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Shipping Conditions
Ice bag
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Product MSDS
https://789.bio/eb/3h3xYKDG
Calculated Molecular Weight
118 kDa
Observed Molecular Weight
140 kDa
Isotype
IgG, κ
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
