TOB1 (phospho Ser164) Antibody

Product Specifications

Background

This gene encodes a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.

NCBI Gene ID

10140

Swiss Prot

P50616

Accession Number

NP_001230806.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

TOB1 (phospho Ser164) antibody was raised against a peptide sequence around phosphorylation site of Serine 164 (A-V-S (p) -P-T) derived from Human TOB1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

40 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of TOB1 only when phosphorylated at serine 164.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

TOB1

NCBI Official Name

Transducer of ERBB2, 1

NCBI Organism

Homo sapiens

Background Reference 01

Matsuda S., Oncogene 12:705-713 (1996) .

Background Reference 02

Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

Background Reference 03

Kalnine N., Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.

Other Product Names

TOB, Transducer of erbB-2 1

Tested Applications

WB, IHC

Protein ID

345197216

Physical Properties

Liquid

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

LH05256V 100 µg

FT1A (FUT6) (NM_001040701) Human Over-expression Lysate

Ask

View Details

126359-FITC 100 µL

EPB41 (Protein 4.1, P4.1, 4.1R, Band 4.1, EPB4.1, E41P) (FITC)

Ask

View Details

M3299-01 20 µL

SPTBN1 (AB1970) Rabbit mAb

Ask

View Details

M3299-02 50 μL

SPTBN1 (AB1970) Rabbit mAb

Ask

View Details

M3299-03 100 µL

SPTBN1 (AB1970) Rabbit mAb

Ask

View Details

M3299-04 200 µL

SPTBN1 (AB1970) Rabbit mAb

Ask

View Details