E2A (phospho Thr355) Antibody

Product Specifications

Background

E2A is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described.

NCBI Gene ID

6929

Swiss Prot

P15923

Accession Number

NP_003191.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E2A (phospho Thr355) antibody was raised against a peptide sequence around phosphorylation site of threonine 355 (P-S-T (p) -P-V) derived from Human E2A .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

67 kDa

Fragment

IgG

Specificity

The antibody detects endogenous levels of E2A only when phosphorylated at threonine 355.

Symbol

TCF3

NCBI Official Name

Transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)

NCBI Organism

Homo sapiens

Background Reference 01

Kamps M.P., Cell 60:547-555 (1990) .

Background Reference 02

Nourse J., Cell 60:535-545 (1990) .

Background Reference 03

Grimwood J., Nature 428:529-535 (2004) .

Other Product Names

E12, Tran, TCF3, TFE2, ITF1

Tested Applications

IHC

Protein ID

27777636

Physical Properties

Liquid

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

MBS7102641 Inquire

Recombinant Thermotoga maritima ATP synthase subunit b (atpF), partial

Ask

View Details

MBS3241955-01 0.1 mg

TMOD4 Peptide - middle region

Ask

View Details

MBS3241955-02 5x 0.1 mg

TMOD4 Peptide - middle region

Ask

View Details

AF8085 50 µL

SUV39H1 Rabbit Polyclonal Antibody

Ask

View Details

MBS6504372-01 0.1 mL

UBE2B, CT (Ubiquitin-conjugating Enzyme E2 B, Ubiquitin-protein Ligase B, Ubiquitin Carrier Protein B, RAD6 Homolog B, HR6B, Ubiquitin-conjugating Enzyme E2-17kD, RAD6B) (MaxLight 650)

Ask

View Details

MBS6504372-02 5x 0.1 mL

UBE2B, CT (Ubiquitin-conjugating Enzyme E2 B, Ubiquitin-protein Ligase B, Ubiquitin Carrier Protein B, RAD6 Homolog B, HR6B, Ubiquitin-conjugating Enzyme E2-17kD, RAD6B) (MaxLight 650)

Ask

View Details