SCF Antibody

Product Specifications

Background

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol-1, 4, 5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

NCBI Gene ID

4254

Swiss Prot

P21583

Accession Number

NP_000890.1

Host

Rabbit

Reactivity

Human

Immunogen

SCF antibody was raised against a peptide sequence around aa.265~269 (E-K-E-R-E) derived from Human SCF.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

25 - 38 kDa

Specificity

This antibody detects endogenous level of total SCF protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

KITLG

NCBI Official Name

KIT ligand

NCBI Organism

Homo sapiens

Background Reference 01

Lu H. S., Clogston C. L., Wypych J., Parker V. P., Lee T. D., Swiderek K., Baltera R. F. Jr., Patel A. C., Chang D. C., Brankow D. W., Liu X. -D., Ogden S. G., Karkare S. B., Hu S. S., Zsebo K. M., Langley K. E. Arch. Biochem. Biophys. 298:150-158 (1992)