SPARC Antibody

Product Specifications

Background

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

NCBI Gene ID

6678

Swiss Prot

P09486

Accession Number

NP_003109.1

Host

Rabbit

Reactivity

Human

Immunogen

SPARC antibody was raised against a peptide sequence around aa.52~56 (V-G-E-F-D) derived from Human SPARC.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

42 kDa

Specificity

This antibody detects endogenous level of total SPARC protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

SPARC

NCBI Official Name

Secreted protein, acidic, cysteine-rich (osteonectin)

NCBI Organism

Homo sapiens

Background Reference 01

Kelm R. J. Jr., Mann K. G. Blood 75:1105-1113 (1990) Wewer U. M., Albrechtsen R., Fisher L. W., Young M. F., Termine J. D. Am. J. Pathol. 132:345-355 (1988) Kuhn C., Mason R. J. Am. J. Pathol. 147:1759-1769 (1995)