Doublecortin Antibody

Product Specifications

Background

Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.

NCBI Gene ID

1641

Swiss Prot

O43602

Accession Number

NP_000546.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Doublecortin antibody was raised against a peptide sequence around aa.413~417 (P-T-S-P-G) derived from Human Doublecortin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience, Stem Cell

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

40 - 45 kDa

Specificity

This antibody detects endogenous level of total Doublecortin protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

DCX

NCBI Official Name

Doublecortin

NCBI Organism

Homo sapiens

Background Reference 01

Des Portes V., Pinard J. -M., Billuart P., Vinet M. -C., Koulakoff A., Carrie A., Gelot A., Dupuis E., Motte J., Berwald-Netter Y., Catala M., Kahn A., Beldjord C., Chelly J. Cell 92:51-61 (1998) Pilz D. T., Matsumoto N., Minnerath S. R., Mills P., Gleeson J. G., Allen K. M., Walsh C. A., Barkovich A. J., Dobyns W. B., Ledbetter D. H., Ross M. E. Hum. Mol. Genet. 7:2029-2037 (1998)