Ring1A Antibody

Product Specifications

Background

Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.

NCBI Gene ID

6015

Swiss Prot

Q06587

Accession Number

NP_002922.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Ring1A antibody was raised against a peptide sequence around aa.35~39 (I-A-V-S-P) derived from Human Ring1A.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

58 kDa

Specificity

This antibody detects endogenous level of total Ring1A protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

RING1

NCBI Official Name

Ring finger protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Cao R., Tsukada Y., Zhang Y. Mol. Cell 20:845-854 (2005) Satijn D. P. E., Gunster M. J., van der Vlag J., Hamer K. M., Schul W., Alkema M. J., Saurin A. J., Freemont P. S., van Driel R., Otte A. P. Mol. Cell. Biol. 17:4105-4113 (1997)