CD22 Antibody

Product Specifications

Background

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2, 6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

NCBI Gene ID

933

Swiss Prot

P20273

Accession Number

NP_001762.2

Host

Rabbit

Reactivity

Human

Immunogen

CD22 antibody was raised against a peptide sequence around aa.839~843 (N-V-D-Y-V) derived from Human CD22.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

140 kDa

Specificity

This antibody detects endogenous level of total CD22 protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

CD22

NCBI Official Name

CD22 molecule

NCBI Organism

Homo sapiens

Background Reference 01

Doody G. M., Justement L. B., Delibrias C. C., Matthews R. J., Lin J., Thomas M. L., Fearon D. T. Science 269:242-244 (1995) Tuscano J. M., Engel P., Tedder T. F., Agarwal A., Kehrl J. H. Eur. J. Immunol. 26:1246-1252 (1996) Law C. L., Sidorenko S. P., Chandran K. A., Zhao Z., Shen S. H., Fischer E. H., Clark E. A. J. Exp. Med. 183:547-560 (1996)