Synaptophysin 2 Antibody

Product Specifications

Background

Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms, and thus can be used as a marker in tumor diagnosis (1) . Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2) . Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3) . Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4) .

NCBI Gene ID

66030

Swiss Prot

P22831

Accession Number

NP_076464.1

Host

Rabbit

Reactivity

Rat

Immunogen

Synaptophysin 2 antibody was raised against a peptide sequence around aa.257~261 (G-P-T-S-F) derived from Rat synaptophysin 2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

37 kDa

Specificity

This antibody detects endogenous levels of total synaptophysin 2 protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

Synpr

NCBI Official Name

Synaptoporin

NCBI Organism

This antibody was raised against a peptide sequence around aa.257~261 (G-P-T-S-F) derived from Rat synaptophysin 2.

Background Reference 01

Wiedenmann, B. et al. (1986) Proc Natl Acad Sci USA 83, 3500-4. Valtorta, F. et al. (2004) Bioessays 26, 445-53. Arthur, C. P. and Stowell, M. H. (2007) Structure 15, 707-14. Bonanomi, D. et al. (2007) Biochem J 404, 525-34.