GRASP Antibody

Product Specifications

Background

GRASP (GRP1-associated scaffold protein, tamalin) is a 395 amino acid proteinencoded by the human gene GRASP . GRASP is a scaffold protein that com-prises multiple protein-interacting domains, including a 95 kDa postsynapticdensity protein (PSD-95) /discs-large/ZO-1 (PDZ) domain, a leucine-zipperregion and a carboxyl-terminal PDZ-binding motif. GRASP is involved withintracellular trafficking and contributes to the macromolecular organizationof group 1 metabotropic glutamate receptors (mGluRs) at synapses. GRASPforms a heteromer composed of GRASP , PSCD2 and at least one mGluR-1. Italso interacts with PSCD3, mGluR-2, mGluR-3 and mGluR-5. GRASP is highlyexpressed in brain and has lower levels of expression in lung, heart, embryo, kidney and ovary.

NCBI Gene ID

56850

Swiss Prot

Q9JHZ4

Accession Number

NP_064522.3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

GRASP antibody was raised against a peptide sequence around aa. 814-818 (Q-E-I-V-R) derived from Rat GRASP.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

95 kDa

Specificity

This antibody detects endogenous level of total GRASP protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

GRIPAP1

NCBI Official Name

GRIP1 associated protein 1

NCBI Organism

This antibody was raised against a peptide sequence around aa. 814-818 (Q-E-I-V-R) derived from Rat GRASP

Background Reference 01

Kitano, J., Kimura, K., Yamazaki, Y., Soda, T., Shigemoto, R., Nakajima, Y. and Nakanishi, S. 2002. Tamalin, J. Neurosci. 22:1280-1289. Hall, B. S., Gabernet-Castello, C., Voak, A., Goulding, D., Natesan, S. K. and Field, M. C. 2006. J. Biol. Chem. 281: 27600-27612. Sugi, T., Oyama, T., Muto, T., Nakanishi, S., Morikawa, K. and Jingami, H. 2007. Crystal structures of autoinhibitory PDZ domain of Tamalin EMBO J. 26: 2192-2205.