CDK4 Antibody

Product Specifications

Background

Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle .

NCBI Gene ID

1019

Swiss Prot

P11802

Accession Number

NP_000066.1

Host

Rabbit

Reactivity

Human

Immunogen

CDK4 antibody was raised against a peptide sequence around aa.295~299 (L-H-K-D-E) derived from Human CDK4. .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

34 kDa

Specificity

This antibody detects endogenous levels of total CDK4 protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

CDK4

NCBI Official Name

Cyclin-dependent kinase 4

NCBI Organism

Homo sapiens

Background Reference 01

Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681. Sherr, C. J. (1996) Science 274, 1672-1677. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925. Gabrielli, B. G. et al. (1999) J Biol Chem 274, 13961-9.