TFE3 Antibody

Product Specifications

Background

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5') . Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.

NCBI Gene ID

7030

Swiss Prot

P19532

Accession Number

NP_006512.2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

TFE3 antibody was raised against a peptide sequence around aa.303~307 (L-D-V-Y-S) derived from Human TFE3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

70 kDa

Specificity

This antibody detects endogenous levels of total TFE3 protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

TFE3

NCBI Official Name

Transcription factor binding to IGHM enhancer 3

NCBI Organism

Homo sapiens

Background Reference 01

Clark J., Lu Y. -J., Sidhar S. K., Parker C., Gill S., Oncogene 15:2233-2239 (1997) Weterman M. A. J., Wilbrink M., Proc. Natl. Acad. Sci. U. S. A. 93:15294-15298 (1996)