OCT-4 Antibody

Product Specifications

Background

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') . Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency

NCBI Gene ID

5460

Swiss Prot

Q01860

Accession Number

NP_002692.2

Host

Rabbit

Reactivity

Human

Immunogen

OCT-4 antibody was raised against a peptide sequence around aa.232~236 (R-K-R-T-S) derived from OCT-4.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

45 kDa

Specificity

This antibody detects endogenous level of total OCT-4 protein.

Applications Notes

Western Blot: 1:1000

Symbol

POU5F1

NCBI Official Name

POU class 5 homeobox 1

NCBI Organism

This antibody was raised against a peptide sequence around aa.232~236 (R-K-R-T-S) derived from OCT-4

Background Reference 01

Pesce, M. and Scholer, H. R. (2001) Stem Cells 19, 271-278. Pan, G. and Thomson, J. A. (2007) Cell Res 17, 42-9. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.