CD8a Antibody [Hit8a] (APC)

Product Specifications

Background

The Hit8a monoclonal antibody reacts with the human CD8a molecule, a 32 kDa cell surface receptor expressed either as a heterodimer (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha) on the majority of thymocytes, a subpopulation of mature T cells, and natural killer cells. CD8 interacts with the major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells. The Hit8a antibody reacts with 13-48% of peripheral lymphocytes, 80% of thymocytes, and a subset of natural killer cells. HIT8a, RPA-T8, and OKT8 antibodies do not compete with each other for binding to peripheral leukocytes, meaning that that they do not recognize the same epitope or block each other by steric hindrance.

NCBI Gene ID

925

Swiss Prot

P01732

Host

Mouse

Reactivity

Human

Clonality

Monoclonal

Clone

Hit8a

Conjugation

APC

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Mouse IgG1, kappa

Specificity

Symbol

CD8A

NCBI Official Name

CD8a molecule

NCBI Organism

Homo sapiens

Background Reference 01

Salem, M. L., Hossain, M. S. (2000) . In vivo acute depletion of CD8+ T cells before murine cytomegalovirus infection upregulated innate antiviral activity of natural killer cells. International journal of immunopharmacology,22 (9), 707-718.

Background Reference 02

Kruisbeek, A. M. (1991) . In Vivo Depletion of CD4 and CD8 Specific T Cells.Current protocols in immunology, 4-1.

Background Reference 03

Davies, A., Kalb, S., Liang, B., Aldrich, C. J., Lemonnier, F. A., Jiang, H., ... Soloski, M. J. (2003) . A peptide from heat shock protein 60 is the dominant peptide bound to Qa-1 in the absence of the MHC class Ia leader sequence peptide Qdm.The Journal of Immunology,170 (10), 5027-5033.