CD86 Antibody [GL-1 (GL1) ]
Product Specifications
Background
The GL-1 monoclonal antibody reacts specifically with the mouse B7-2 (also known as CD86), a costimulatory molecule expressed by B and T lymphocytes, macrophages (thioglycollate-induced from peritoneum), astrocytes and dendritic cells. The memory CD4+ T lymphocytes express CD86 (as mRNA and protein) . CD86, and the B7-1 (CD 80) molecule, are ligands for CD152 and CD28, and influence the costimulatory interactions between lymphocytes B and T. B7-2 is also involved in the mouse natural killer cell-mediated cytotoxicity. The GL-1 antibody blocks the mixed lymphocyte reaction (MLR) and inhibits the T-cells stimulation by antigen-presenting cells. Mixtures of anti-B7-1 antibody and GL-1 were reported to inhibit the interaction between CD125 and its ligand with the in vivo priming of cytotoxic T lymphocytes.
NCBI Gene ID
12524
Swiss Prot
P42082
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Clone
GL-1 (GL1)
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Innate Immunity
Purification
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Concentration
Batch dependent
Buffer
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=76-597
Fragment
Rat IgG2a, kappa
Specificity
The GL-1 monoclonal antibody reacts specifically with the mouse B7-2 (also known as CD86), a costimulatory molecule expressed by B and T lymphocytes, macrophages (thioglycollate-induced from peritoneum), astrocytes and dendritic cells.
Symbol
Cd86
NCBI Official Name
CD86 antigen
NCBI Organism
Mus musculus
Background Reference 01
Hathcock, K. S., Laszlo, G., Dickler, H. B., Bradshaw, J., Linsley, P., Hodes, R. J. (1993) . Identification of an alternative CTLA-4 ligand costimulatory for T cell activation.Science,262 (5135), 905-907.
Background Reference 02
Inaba, K., Witmer-Pack, M., Inaba, M., Hathcock, K. S., Sakuta, H., Azuma, M., ... Steinman, R. M. (1994) . The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro.The Journal of experimental medicine,180 (5), 1849-1860.
Background Reference 03
Hathcock, K. S., Laszlo, G., Pucillo, C., Linsley, P., Hodes, R. J. (1994) . Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function.The Journal of experimental medicine,180 (2), 631-640.
Other Product Names
B7, B70, MB7, B7-2, B7.2, CLS1, Ly58, ETC-1, Ly-58, MB7-2, Cd28l2, TS/A-2, Cd86
Tested Applications
Flow, Functional Assays, IHC, IF
Physical Properties
Liquid
Curated Selection
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