CD40 Antibody [5C3]

Product Specifications

Background

The 5C3 monoclonal antibody specifically reacts with human CD40, a 48kDA type I glycoprotein. CD40 is part of the TNFR family and expressed on a subset of T cells, B cells, dendritic cells, and macrophages. It has been shown to be involved in the protection of B cells from apoptosis, B cell differentiation, co-stimulation, and isotype class-switching. The 5C3 antibody partially blocks the binding of CD40L (CD154) . In the presence of IL-4 or PMA, the 5C3 antibody promotes B cell proliferation.

NCBI Gene ID

958

Swiss Prot

P25942

Host

Mouse

Reactivity

Human

Clonality

Monoclonal

Clone

5C3

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C . Do not freeze.

Fragment

Mouse IgG1, kappa

Specificity

The 5C3 monoclonal antibody specifically reacts with human CD40, a 48kDA type I glycoprotein.

Symbol

CD40

NCBI Official Name

CD40 molecule, TNF receptor superfamily member 5

NCBI Organism

Homo sapiens

Background Reference 01

Schlossman, S. F. (1995) .Leucocyte typing V: White cell differentiation antigens: Proceedings of the Fifth International Workshop and Conference, Held in Boston, USA 3-7 November, 1993. Oxford University Press.

Background Reference 02

Barclay, A. N., Birkeland, M. L., Brown, M. H., Beyers, A. D., David, S. J., Somoza, C., Williams, A. F. The Leukocyte Antigen Facts Book. 1993.Academic Prss, London.

Background Reference 03

Challa, A., Pound, J. D., Gordon, J., Armitage, R. J. (1999) . Epitope‐dependent synergism and antagonism between CD40 antibodies and soluble CD40 ligand for the regulation of CD23 expression and IgE synthesis in human B cells.Allergy,54 (6), 576-583.