EIF2a (Ab 51) Antibody

Product Specifications

Background

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

NCBI Gene ID

1965

Swiss Prot

P05198

Accession Number

NP_004085.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

EIF2a (Ab-51) antibody was raised against a peptide sequence around aa.49~53 (E-L-S-R-R) derived from Human eIF2α .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

38 kDa

Specificity

This antibody detects endogenous level of total eIF2α protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

EIF2S1

NCBI Official Name

Eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa

NCBI Organism

Homo sapiens

Background Reference 01

Xavier Saelens, et, al. (2001) J. Biol. Chem; 276: 41620 - 41628. Hong-Li Wu, Yu-Hua Li, Yan-Hua Lin, et al. (2008) Salvianolic acid B protects human endothelial cells from oxidative stress damage: a possible protective role of glucose-regulated protein 78 induction Cardiovasc Res doi:10. 1093/cvr/cvn262.