EIF4E (phospho Ser209) Antibody

Product Specifications

Background

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

NCBI Gene ID

1977

Swiss Prot

P06730

Accession Number

NP_001124150.1

Host

Rabbit

Reactivity

Human

Immunogen

EIF4E (Phospho-Ser209) antibody was raised against a peptide sequence around phosphorylation site of serine 209 (S-G-S (p) -T-T) derived from Human eIF4E.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

25 kDa

Specificity

This antibody detects endogenous level of eIF4E only when phosphorylated at serine 209.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

EIF4E

NCBI Official Name

Eukaryotic translation initiation factor 4E

NCBI Organism

Homo sapiens

Background Reference 01

Li BD, et al. (1998) Ann Surg; 227 (5) : 756-763Altmann M, et al. (1989) Nucleic Acids Res; 17 (18) : 7520De Gregorio E, et al. (2001) RNA; 7 (1) : 106-113Gu W, et al. (2004) Nucleic Acids Res; 32 (15) : 4448-4461