CPSF4 Antibody

Product Specifications

Background

Inhibition of the nuclear export of poly (A) -containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs.

NCBI Gene ID

10898

Swiss Prot

O95639

Accession Number

O95639

Host

Rabbit

Reactivity

Human

Immunogen

This CPSF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-123 amino acids from the Central region of human CPSF4.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Rat, Xenopus

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

30 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

CPSF4

NCBI Official Name

Cleavage and polyadenylation specificity factor subunit 4

NCBI Organism

Homo sapiens

Background Reference 01

Twu, K.Y., et al. J. Virol. 81 (15) :8112-8121 (2007)

Background Reference 02

Oh, J.H., et al. Mamm. Genome 16 (12) :942-954 (2005)

Background Reference 03

Kaufmann, I., et al. EMBO J. 23 (3) :616-626 (2004)

Background Reference 04

De Vries, H., et al. EMBO J. 19 (21) :5895-5904 (2000)

Other Product Names

Cleavage and polyadenylation specificity factor subunit 4, Cleavage and polyadenylation specificity factor 30 kDa subunit, CPSF 30 kDa subunit, NS1 effector domain-binding protein 1, Neb-1, No arches homolog, CPSF4, CPSF30, NAR, NEB1