COT Antibody

Product Specifications

Background

COT, a member of the MAPKKK subfamily of Ser/Thr protein kinases, is able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. It plays a role in the cell cycle. The longer form of cot has some transforming activity, although it is much weaker than the activated cot oncoprotein. This cytoplasmic protein is expressed in several normal tissues and human tumor-derived cell lines. The 58 kDa form is activated specifically during the S and G2/M phases of the cell cycle. The longer form undergoes phosphorylation on Ser residues mainly, and the shorter form on both Ser and Thr residues.

NCBI Gene ID

1326

Swiss Prot

P41279

Accession Number

P41279

Host

Rabbit

Reactivity

Human

Immunogen

This COT (MAP3K8/MEKK8) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 368-397 amino acids from the C-terminal region of human COT (MAP3K8/MEKK8) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Signal Transduction

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

53 kDa

Fragment

Rabbit Ig

Symbol

MAP3K8

NCBI Official Name

Mitogen-activated protein kinase kinase kinase 8

NCBI Organism

Homo sapiens

Background Reference 01

Sanchez-Gongora, E., et al., J. Biol. Chem. 275 (40) :31379-31386 (2000) .

Background Reference 02

Aoki, M., et al., J. Biol. Chem. 268 (30) :22723-22732 (1993) .

Background Reference 03

Chan, A.M., et al., Oncogene 8 (5) :1329-1333 (1993) .

Background Reference 04

Miyoshi, J., et al., Mol. Cell. Biol. 11 (8) :4088-4096 (1991) .

Other Product Names

Mitogen-activated protein kinase kinase kinase 8, Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2, TPL-2, MAP3K8, COT, ESTF