E2F1 Antibody

Product Specifications

Background

E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another two members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

NCBI Gene ID

1869

Swiss Prot

Q01094

Accession Number

Q01094

Host

Rabbit

Reactivity

Human

Immunogen

This E2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310-344 amino acids from human E2F1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

47 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

E2F1

NCBI Official Name

Transcription factor E2F1

NCBI Organism

Homo sapiens

Background Reference 01

O'Donnell, K.A., et al., Nature 435 (7043) :839-843 (2005) .

Background Reference 02

Wang, C., et al., J. Biol. Chem. 280 (13) :12339-12343 (2005) .

Background Reference 03

Joshi, B., et al., Oncogene 24 (13) :2204-2217 (2005) .

Background Reference 04

Saberwal, G., et al., Int. J. Hematol. 80 (2) :146-154 (2004) .

Other Product Names

Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1, E2F1, RBBP3