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SRD5A2 Antibody

Product Specifications

Background

This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH) .

NCBI Gene ID

6716

Swiss Prot

P31213

Accession Number

P31213

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This SRD5A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-182 amino acids from the Central region of human SRD5A2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle, Obesity, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

28 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

SRD5A2

NCBI Official Name

3-oxo-5-alpha-steroid 4-dehydrogenase 2

NCBI Organism

Homo sapiens

Background Reference 01

Thigpen, A.E., et al. N. Engl. J. Med. 327 (17) :1216-1219 (1992)

Background Reference 02

Thigpen, A.E., et al. J. Clin. Invest. 90 (3) :799-809 (1992)

Background Reference 03

Labrie, F., et al. Endocrinology 131 (3) :1571-1573 (1992)

Background Reference 04

Andersson, S., et al. Nature 354 (6349) :159-161 (1991)

Other Product Names

3-oxo-5-alpha-steroid 4-dehydrogenase 2, 5 alpha-SR2, SR type 2, Steroid 5-alpha-reductase 2, S5AR 2, Type II 5-alpha reductase, SRD5A2

Tested Applications

WB

Protein ID

401056

Physical Properties

Liquid

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