MTHFD1 Antibody

Product Specifications

Background

MTHFD1 is a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.

NCBI Gene ID

4522

Swiss Prot

P11586

Accession Number

P11586

Host

Rabbit

Reactivity

Human

Immunogen

This MTHFD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 535-562 amino acids from the Central region of human MTHFD1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle, Obesity, Signal Transduction

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

102 kDa

Fragment

Rabbit Ig

Symbol

MTHFD1

NCBI Official Name

C-1-tetrahydrofolate synthase, cytoplasmic

NCBI Organism

Homo sapiens

Background Reference 01

Ivanov, A., Am Diet Assoc 109 (2), 313-318 (2009)

Background Reference 02

Schmidt A., Biochemistry 39:6325-6335 (2000)

Background Reference 03

Hol F.A., Clin. Genet. 53:119-125 (1998)

Other Product Names

C-1-tetrahydrofolate synthase, cytoplasmic, C1-THF synthase, Methylenetetrahydrofolate dehydrogenase, Methenyltetrahydrofolate cyclohydrolase, Formyltetrahydrofolate synthetase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, MTHFD1, MTHFC, MTHFD