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Vinculin

Product Specifications

Background

Actin filament (F-actin) -binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity) .

NCBI Gene ID

22330

Swiss Prot

Q64727

Accession Number

Q64727

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 903-937 amino acids from mouse.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

117 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:2000

Symbol

Vcl

NCBI Official Name

Vinculin

NCBI Organism

Mus musculus

Background Reference 01

Coll J.-L., et al.Proc. Natl. Acad. Sci. U.S.A. 92:9161-9165 (1995) .

Background Reference 02

Alatortsev V.E., et al.FEBS Lett. 413:197-201 (1997) .

Background Reference 03

Carninci P., et al.Science 309:1559-1563 (2005) .

Background Reference 04

Lubec G., et al.Submitted (JAN-2009) to UniProtKB.

Other Product Names

Vinculin, Metavinculin, Vcl

Tested Applications

WB

Physical Properties

Liquid

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