PPM1F Antibody

Product Specifications

Background

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.

NCBI Gene ID

9647

Swiss Prot

P49593

Accession Number

P49593

Host

Rabbit

Reactivity

Human

Immunogen

This PPM1F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-303 amino acids from the Central region of human PPM1F.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

50 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

PPM1F

NCBI Official Name

Protein phosphatase 1F

NCBI Organism

Homo sapiens

Background Reference 01

Harvey, B.P., et al. J. Biol. Chem. 279 (23) :24889-24898 (2004)

Background Reference 02

Koh, C.G., et al. Curr. Biol. 12 (4) :317-321 (2002)

Background Reference 03

Tan, K.M., et al. J. Biol. Chem. 276 (47) :44193-44202 (2001)

Background Reference 04

Kitani, T., et al. J. Biochem. 125 (6) :1022-1028 (1999)

Other Product Names

Protein phosphatase 1F, Ca (2+) /calmodulin-dependent protein kinase phosphatase, CaM-kinase phosphatase, CaMKPase, Partner of PIX 2, Protein fem-2 homolog, hFem-2, PPM1F, KIAA0015, POPX2