RNF144B Antibody

Product Specifications

Background

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.

NCBI Gene ID

255488

Swiss Prot

Q7Z419

Accession Number

Q7Z419

Host

Rabbit

Reactivity

Human

Immunogen

This RNF144B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-118 amino acids from the Central region of human RNF144B.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

34 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

RNF144B

NCBI Official Name

E3 ubiquitin-protein ligase RNF144B

NCBI Organism

Homo sapiens

Background Reference 01

Sayan, B.S., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (29) :12877-12882 (2010)

Background Reference 02

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

Background Reference 03

Benard, G., et al. EMBO J. 29 (8) :1458-1471 (2010)

Background Reference 04

Markson, G., et al. Genome Res. 19 (10) :1905-1911 (2009)

Other Product Names

E3 ubiquitin-protein ligase RNF144B, 632-, IBR domain-containing protein 2, RING finger protein 144B, p53-inducible RING finger protein, RNF144B, IBRDC2, P53RFP