SEPSECS Antibody

Product Specifications

Background

The 21st amino acid, selenocysteine (sec), is distinct from other amino acids because it lacks its own tRNA synthetase and is the only amino acid synthesized on its cognate tRNA. Synthesis of sec begins with acylation of tRNA (sec) (TRSP; MIM 165060) by seryl-tRNA synthetase (SARS; MIM 607529) to give ser-tRNA (sec), which is subsequently phosphorylated by O-phosphoseryl-tRNA kinase (PSTK; MIM 611310) to give O-phosphoseryl-tRNA (sec) . SEPSECS catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA (sec) to selenocysteinyl-tRNA (sec) using selenophosphate as the selenium donor (Palioura et al., 2009 [PubMed 19608919]) .

NCBI Gene ID

51091

Swiss Prot

Q9HD40

Accession Number

Q9HD40

Host

Rabbit

Reactivity

Human

Immunogen

This SEPSECS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-474 amino acids from the C-terminal region of human SEPSECS.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

56 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

SEPSECS

NCBI Official Name

O-phosphoseryl-tRNA (Sec) selenium transferase

NCBI Organism

Homo sapiens

Background Reference 01

Agamy, O., et al. Am. J. Hum. Genet. 87 (4) :538-544 (2010)

Background Reference 02

Hart, K., et al. Lung Cancer (2010) In press :

Background Reference 03

Volkmann, M., et al. J. Autoimmun. 34 (1) :59-65 (2010)

Background Reference 04

Palioura, S., et al. Science 325 (5938) :321-325 (2009)

Other Product Names

O-phosphoseryl-tRNA (Sec) selenium transferase, Liver-pancreas antigen, LP, SLA-p35, SLA/LP autoantigen, Selenocysteine synthase, Sec synthase, Selenocysteinyl-tRNA (Sec) synthase, Sep-tRNA:Sec-tRNA synthase, SepSecS, Soluble liver antigen, SLA, UGA suppressor tRNA-associated protein, tRNA (Ser/Sec) -associated antigenic protein, SEPSECS, TRNP48