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DPH2 Antibody

Product Specifications

Background

This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.

NCBI Gene ID

1802

Swiss Prot

Q9BQC3

Accession Number

Q9BQC3

Host

Rabbit

Reactivity

Human

Immunogen

This DPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-148 amino acids from the N-terminal region of human DPH2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

52 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

DPH2

NCBI Official Name

Diphthamide biosynthesis protein 2

NCBI Organism

Homo sapiens

Background Reference 01

Rose, J. Phd, et al. Mol. Med. (2010) In press :

Background Reference 02

Liu, S., et al. Mol. Cell. Biol. 24 (21) :9487-9497 (2004)

Background Reference 03

Schultz, D.C., et al. Genomics 52 (2) :186-191 (1998)

Background Reference 04

Foley, B.T., et al. J. Biol. Chem. 270 (39) :23218-23225 (1995)

Other Product Names

Diphthamide biosynthesis protein 2, DPH2 homolog, HsDph2, Diphthamide biosynthesis protein 2 homolog-like 2, DPH2-like 2, DPH2, DPH2L2

Tested Applications

WB

Protein ID

74761201

Physical Properties

Liquid

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