DPH1 Antibody

Product Specifications

Background

Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610) . This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]) .[supplied by OMIM].

NCBI Gene ID

1801

Swiss Prot

Q9BZG8

Accession Number

Q9BZG8

Host

Rabbit

Reactivity

Human

Immunogen

This DPH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 384-412 amino acids from the C-terminal region of human DPH1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

49 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

DPH1

NCBI Official Name

Diphthamide biosynthesis protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Liu, S., et al. Mol. Cell. Biol. 24 (21) :9487-9497 (2004)

Background Reference 02

Cardoso, C., et al. Am. J. Hum. Genet. 72 (4) :918-930 (2003)

Background Reference 03

Chen, C.M., et al. Biochem. Biophys. Res. Commun. 286 (5) :1019-1026 (2001)

Background Reference 04

Salicioni, A.M., et al. Genomics 69 (1) :54-62 (2000)

Other Product Names

Diphthamide biosynthesis protein 1, DPH1 homolog, HsDph1, Diphthamide biosynthesis protein 2 homolog-like 1, DPH-like 1, DPH2-like 1, Diphthamide biosynthesis protein 2-like, Ovarian cancer-associated gene 1 protein, DPH1, DPH2L, DPH2L1, OVCA1